Chemerin ELISA Kit

Catalog No. ABIN6720010

Supplier Product No.: koa0798

Human Chemerin ELISA Kit, Colorimetric assay for quantification of Human Chemerin.

Quick Overview for Chemerin ELISA Kit (ABIN6720010)

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details Chemerin ELISA Kit

Minimum Detection Limit: 78 pg/mL

Supplier Product No.: koa0798

Supplier: Rockland

Purpose: Human Chemerin - RARRES2 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: V17-S163

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 20 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Chemerin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Chemerin standard solution and each sample is measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 4.7%, 6.4%, 7.6%
Inter-Assay Precision (CV%): 6%, 8.1%, 9.3%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Chemerin

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Alternative Name: Chemerin/RARRES2

Background:

Background: Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

Gene Full Name: retinoic acid receptor responder 2

Gene ID: 5919

NCBI Accession: NP_002880

UniProt: Q99969

Pathways: Brown Fat Cell Differentiation