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Chemerin ELISA Kit

This Mouse Chemerin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Chemerin.
Rockland
Catalog No. ABIN6720015
Supplier Product No.: koa0799

Quick Overview for Chemerin ELISA Kit (ABIN6720015)

Target

See all Chemerin (RARRES2) ELISA Kits
Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Reactivity

  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.78 ng/mL - 50 ng/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
  • Minimum Detection Limit

    0.78 ng/mL

    Supplier Product No.

    koa0799

    Supplier

    Rockland

    Purpose

    Mouse Chemerin - RARRES2 Sandwich ELISA Kit for Quantitative Detection

    Brand

    AccuSignal™

    Analytical Method

    Quantitative

    Specificity

    Production: Natural and recombinant mouse Chemerin. There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 20 pg/mL

    Components

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Application Notes

    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Chemerin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse Chemerin standard solution and each sample is measured in duplicate.

    Comment

    Standard: Expression system for standard: E.coli, Immunogen sequence: T20-K162

    Sample Volume

    100 μL

    Plate

    Pre-coated

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Expiry Date

    12 months
  • Target See all Chemerin (RARRES2) ELISA Kits

    Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

    Alternative Name

    Chemerin/RARRES2

    Background

    Synonyms: chemerin, HP10433, RAR-responsive protein TIG2, RARRES2, retinoic acid receptor responder (tazarotene induced) 2, retinoic acid receptor responder protein 2, tazarotene-induced gene 2 protein, TIG3

    Background: Chemerin, also known as RARRES2 or TIG2, is a protein that is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1(also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What's more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.

    Gene ID

    71660

    NCBI Accession

    NP_082128

    UniProt

    Q9DD06

    Pathways

    Brown Fat Cell Differentiation
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