PLTP ELISA Kit

Catalog No. ABIN6720017

Product Details PLTP ELISA Kit

Target: PLTP (Phospholipid Transfer Protein (PLTP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: BPI fold containing family E, BPIFE, HDLCQ9, High density lipoprotein cholesterol level quantitative trait locus 9 included, Lipid transfer protein II, OD107, Phospholipid transfer protein, Plasma phospholipid transfer protein, Pltp, PLTP_HUMAN

Background: Phospholipid transfer protein (PLTP), also known as lipid transfer protein II is a protein that in humans is encoded by the PLTP gene. This gene is mapped to 20q13.12. The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene.

Gene Name: PLTP

Production: Natural and recombinant mouse PLTP. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E18-A493

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse PLTP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse PLTP standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for PLTP

Target: PLTP (Phospholipid Transfer Protein (PLTP))

Alternative Name: PLTP (PLTP Products)

Synonyms: zgc:100903 ELISA Kit, MGC84445 ELISA Kit, PLTP ELISA Kit, hdlcq9 ELISA Kit, DKFZp469A1437 ELISA Kit, BPIFE ELISA Kit, HDLCQ9 ELISA Kit, Bpife ELISA Kit, OD107 ELISA Kit, phospholipid transfer protein ELISA Kit, phospholipid transfer protein S homeolog ELISA Kit, PLTP ELISA Kit, pltp ELISA Kit, pltp.S ELISA Kit, LOC542343 ELISA Kit, Pltp ELISA Kit

Gene ID: 18830

NCBI Accession: NP_035255

UniProt: P55065

Pathways: Lipid Metabolism