CRP ELISA Kit

Catalog No. ABIN6720020

Supplier Product No.: koa0789

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CRP.

Quick Overview for CRP ELISA Kit (ABIN6720020)

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CRP ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Supplier Product No.: koa0789

Supplier: Rockland

Purpose: Human CRP Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CRP. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 1.56 ng/mL-100 ng/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CRP standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: F17-P224

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: CRP

Background:

Synonyms: C reactive protein pentraxin related, C-reactive protein, C-reactive protein(1-205), CRP, CRP_HUMAN, MGC149895, MGC88244, Pentraxin 1, Pentraxin 1 short, PTX 1, PTX1

Background: C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4 % of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

Gene ID: 1401

NCBI Accession: NP_000558

UniProt: P02741

Pathways: Carbohydrate Homeostasis