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NBL1 ELISA Kit

NBL1 Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6720028
  • Target See all NBL1 ELISA Kits
    NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))
    Reactivity
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125 pg/mL - 8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: D1S1733E, DAN, DAN domain family member 1, DAND 1, DAND1, Differential screening selected gene aberrant in neuroblastoma, MGC8972, NB, NBL 1, Neuroblastoma candidate region suppression of tumorigenicity 1, Neuroblastoma suppression of tumorigenicity 1, Neuroblastoma suppressor of tumorigenicity 1, NO 3, NO3, Zinc finger protein DAN

    Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

    Gene Name: NBL1

    Production: Natural and recombinant human DAN. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: A17-D181

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    Discover our top product NBL1 ELISA Kit
  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DAN standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 125pg/mL-8000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all NBL1 ELISA Kits
    NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))
    Alternative Name
    DAN - NBL1 (NBL1 Products)
    Synonyms
    D1S1733E ELISA Kit, DAN ELISA Kit, DAND1 ELISA Kit, NB ELISA Kit, NO3 ELISA Kit, D4H1S1733E ELISA Kit, Dana ELISA Kit, dand1 ELISA Kit, nbl1-a ELISA Kit, no3 ELISA Kit, 4.1N ELISA Kit, Epb41l1 ELISA Kit, NBL1 ELISA Kit, mKIAA0338 ELISA Kit, C1orf151-NBL1 ELISA Kit, dan ELISA Kit, zgc:77251 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist ELISA Kit, neuroblastoma, suppression of tumorigenicity 1 ELISA Kit, neuroblastoma 1, DAN family BMP antagonist L homeolog ELISA Kit, neuroblastoma suppressor of tumorigenicity 1 ELISA Kit, erythrocyte membrane protein band 4.1 like 1 ELISA Kit, MINOS1-NBL1 readthrough ELISA Kit, NBL1 ELISA Kit, Nbl1 ELISA Kit, nbl1.L ELISA Kit, LOC535280 ELISA Kit, Epb41l1 ELISA Kit, MINOS1-NBL1 ELISA Kit, nbl1 ELISA Kit
    Gene ID
    4681
    NCBI Accession
    NP_001191013
    UniProt
    P41271
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