NBL1 ELISA Kit

Catalog No. ABIN6720028

Supplier Product No.: koa0438

Human NBL1 ELISA Kit, Colorimetric assay for quantification of Human NBL1.

Quick Overview for NBL1 ELISA Kit (ABIN6720028)

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 125 pg/mL - 8000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details NBL1 ELISA Kit

Minimum Detection Limit: 125 pg/mL

Supplier Product No.: koa0438

Supplier: Rockland

Purpose: Human DAN - NBL1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human DAN. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL human DAN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DAN standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: A17-D181

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for NBL1

Target: NBL1 (Neuroblastoma 1, DAN Family BMP Antagonist (NBL1))

Alternative Name: DAN/NBL1

Background:

Synonyms: D1S1733E, DAN, DAN domain family member 1, DAND 1, DAND1, Differential screening selected gene aberrant in neuroblastoma, MGC8972, NB, NBL 1, Neuroblastoma candidate region suppression of tumorigenicity 1, Neuroblastoma suppression of tumorigenicity 1, Neuroblastoma suppressor of tumorigenicity 1, NO 3, NO3, Zinc finger protein DAN

Background: Differential screening-selected gene aberrative in neuroblastoma (DAN) is a member of the DAN family of secreted glycoproteins that are putative BMP antagonists. The NBL1 gene, also known as DAN, is originally cloned from a normal rat fibroblast cDNA library by a differential screening method. The human DAN gene is mapped to chromosome 1p36.13-p36. It is found that the DAN gene possesses a tumor suppressive activity when overexpressed in v-src transformed cells.

Gene ID: 4681

NCBI Accession: NP_001191013

UniProt: P41271