CCL14 ELISA Kit

Catalog No. ABIN6720037

Product Details CCL14 ELISA Kit

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: C-C motif chemokine 14,CC-1, CC-3, Chemokine (C-C motif) ligand 14, Chemokine CC-1/CC-3, Chemokine CC-3, CKB1, HCC-1, HCC-1(1-74), HCC-1/HCC-3, HCC-3, MCIF, NCC-2, NCC2, New CC chemokine 2, SCYA14, SCYL2, Small inducible cytokine subfamily A (Cys-Cys) member 14, Small-inducible cytokine A14, SY14

Background: Chemokine(C-C motif) ligand 14(CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46 % identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Gene Name: CCL14

Production: Natural and recombinant human CCL14. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: T20-N93

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human CCL14 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CCL14 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CCL14

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Alternative Name: CCL14 - HCC-1 (CCL14 Products)

Synonyms: CCL14 ELISA Kit, CC-1 ELISA Kit, CC-3 ELISA Kit, CKB1 ELISA Kit, HCC-1 ELISA Kit, HCC-1(1-74) ELISA Kit, HCC-1/HCC-3 ELISA Kit, HCC-3 ELISA Kit, MCIF ELISA Kit, NCC-2 ELISA Kit, NCC2 ELISA Kit, SCYA14 ELISA Kit, SCYL2 ELISA Kit, SY14 ELISA Kit, C-C motif chemokine ligand 14 ELISA Kit, C-C motif chemokine 14 ELISA Kit, chemokine (C-C motif) ligand 14 ELISA Kit, CCL14 ELISA Kit, Ccl14 ELISA Kit, LOC480603 ELISA Kit, LOC101115044 ELISA Kit

Gene ID: 6358

NCBI Accession: NP_116738

UniProt: Q16627