IL-18 ELISA Kit

Catalog No. ABIN6720050

Supplier Product No.: koa0523

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-18.

Quick Overview for IL-18 ELISA Kit (ABIN6720050)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details IL-18 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0523

Supplier: Rockland

Purpose: Human IL-18 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human IL-18. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 15.6pg/mL-1000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human IL-18 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-18 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: Y33-D197

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL-18

Background:

Synonyms: Iboctadekin, IFN gamma inducing factor, IFN-gamma-inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL-1 gamma, IL-18, IL1 gamma, IL18, IL18 protein, IL18_HUMAN, IL1F4, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interferon gamma-inducing factor, Interleukin 1 gamma, Interleukin 18, Interleukin-1 gamma, Interleukin-18, Interleukin18, MGC12320

Background: Interleukin(IL)-18, also called Interferon-gamma-inducing factor(IGIF), augments natural killer(NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1(Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

Gene ID: 3606

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome