Angiopoietin 1 ELISA Kit

Catalog No. ABIN6720078

Supplier Product No.: koa0770

This Mouse Angiopoietin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Angiopoietin 1 in Cell Culture Supernatant, Plasma (EDTA - heparin) and Serum.

Quick Overview for Angiopoietin 1 ELISA Kit (ABIN6720078)

Target: Angiopoietin 1 (ANGPT1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details Angiopoietin 1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0770

Supplier: Rockland

Purpose: Mouse Angiopoietin-1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse Angiopoietin-1. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Angiopoietin-1 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: H16-F497

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: Angiopoietin-1

Background:

Synonyms: 1110046O21Rik, AGP 1, AGP1, AGPT, ANG 1, ANG1, Angiopoietin1, ANGPT 1, ANGPT1, KIAA0004

Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Gene ID: 11600

NCBI Accession: NP_033770

UniProt: O08538

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process