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CCL28 ELISA Kit

CCL28 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Catalog No. ABIN6720154
  • Target See all CCL28 ELISA Kits
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    Reactivity
    • 6
    • 3
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    31.2 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Application
    ELISA
    Purpose
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Characteristics

    Synonyms: CCK 1, CCK1, CCL 28, Chemokine (C-C motif) ligand 28, MEC, MGC71902, Mucosae associated epithelial chemokine, OTTHUMP0000022162, SCYA28, Small inducible cytokine A28, Small inducible cytokine A28 [Precursor], Small inducible cytokine subfamily A (Cys-Cys), member 28

    Background: CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

    Gene Name: CCL28

    Production: Natural and recombinant human CCL28. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: I23-Y127

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    Discover our top product CCL28 ELISA Kit
  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL28 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Sample Volume
    100 μL
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Target See all CCL28 ELISA Kits
    CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
    Alternative Name
    CCL28 (CCL28 Products)
    Synonyms
    Scya28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, nrpb ELISA Kit, enc-1 ELISA Kit, pig10 ELISA Kit, Encr-3 ELISA Kit, klhl35 ELISA Kit, Xencr-3 ELISA Kit, tp53i10 ELISA Kit, LOC100303767 ELISA Kit, CCK1 ELISA Kit, MEC ELISA Kit, SCYA28 ELISA Kit, C-C motif chemokine ligand 28 ELISA Kit, ectodermal-neural cortex 1 (with BTB domain) ELISA Kit, C-C motif chemokine 28 ELISA Kit, chemokine (C-C motif) ligand 28 ELISA Kit, Ccl28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, enc1 ELISA Kit, LOC100303767 ELISA Kit
    Gene ID
    65477
    NCBI Accession
    NP_001288802
    UniProt
    Q9NRJ3
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