DIABLO ELISA Kit

Catalog No. ABIN6720163

Product Details DIABLO ELISA Kit

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: DFNA64, DIABLO, diablo homolog, mitochondrial, diablo, IAP-binding mitochondrial protein, DIABLO-S, direct IAP-binding protein with low pI, mitochondrial Smac protein, second mitochondria-derived activator of caspase, SMAC3

Background: Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases(SMAC), is a protein that in humans is encoded by the DIABLO gene(direct IAP binding protein with low pI). It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A(MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein(IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.

Gene Name: DIABLO

Production: Natural and recombinant human Diablo. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A56-D239

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Diablo standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Diablo standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for DIABLO

Target: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Alternative Name: Diablo - SMAC (DIABLO Products)

Synonyms: smac ELISA Kit, smac3 ELISA Kit, diablo-s ELISA Kit, 0610041G12Rik ELISA Kit, 1700006L01Rik ELISA Kit, AU040403 ELISA Kit, Smac ELISA Kit, DFNA64 ELISA Kit, SMAC ELISA Kit, DBO ELISA Kit, XSmac ELISA Kit, diablo, IAP-binding mitochondrial protein ELISA Kit, diablo IAP-binding mitochondrial protein ELISA Kit, diablo, IAP-binding mitochondrial protein L homeolog ELISA Kit, diablo ELISA Kit, Diablo ELISA Kit, DIABLO ELISA Kit, diablo.L ELISA Kit

Gene ID: 56616

NCBI Accession: NP_001265231

UniProt: Q9NR28

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity