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Nephroblastoma Overexpressed (NOV) ELISA Kit ELISA Kit

NOV Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant
Catalog No. ABIN6720176
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1 kit
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  • Target
    Detection Method
    Method Type
    Sandwich ELISA
    Detection Range
    31.2 pg/mL - 2000 pg/mL
    Minimum Detection Limit
    31.2 pg/mL
    Sandwich ELISA for Quantitative Detection of Antigen
    Sample Type
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method

    Synonyms: CCN family member 3, CCN3, IBP-9, IGF-binding protein 9, IGFBP-9, IGFBP9, Insulin-like growth factor-binding protein 9, Nephroblastoma overexpressed, Nephroblastoma overexpressed gene protein homolog, Nephroblastoma-overexpressed gene protein homolog, NOV, NOV_HUMAN, NovH, Protein NOV homolog

    Background: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

    Gene Name: NOV

    Production: Natural and recombinant human NOV. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: NSO, Immunogen sequence: T32-M357

  • Target
    Alternative Name
    NOV - CCN3 (NOV ELISA Kit Abstract)
    Gene ID
    NCBI Accession
    Smooth Muscle Cell Migration, Growth Factor Binding
  • Application Notes

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NOV standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human NOV standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Other Performance Data: DOE: 2-8 C - 11/23/17 -20 C - 05/24/18

    Sample Volume
    100 μL
    For Research Use only
  • Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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