Stanniocalcin 1 ELISA Kit

Catalog No. ABIN6720178

Product Details Stanniocalcin 1 ELISA Kit

Target: Stanniocalcin 1 (STC1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Stanniocalcin, Stanniocalcin-1, STC, STC-1, STC1, STC1_HUMAN

Background: Stanniocalcin-1 is a glycoprotein which is encoded by the STC1 gene. This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. Human Stanniocalcin-1 is a putative molecular biomarker of leukemic microenvironment and the only molecular function known up to date is a SUMO E3 ligase activity in the SUMOylation cycle. STC1 interacts with lots of proteins in the cytoplasm, mitochondria, endoplasmatic reticulum and dot-like fashion in the nucleus. The N-terminal region of STC1 is the function region which is responsible to establish the interaction with its partners, including SUMO1. Low resolution studies shows that STC1 is an anti-parallel homodimer in solution and the cystein 202 is responsible for the dimerization of this protein. All the 5 dissulfide bonds of human STC1 are conserved and have the same profile of fish STC.

Gene Name: STC1

Production: Natural and recombinant human STC1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V34-A247

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human STC1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma (heparin, EDTA) to each empty well. It is recommended that each human STC1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Stanniocalcin 1

Target: Stanniocalcin 1 (STC1)

Alternative Name: Stanniocalcin 1 - STC1 (STC1 Products)

Synonyms: STC ELISA Kit, Stc ELISA Kit, stc1 ELISA Kit, MGC82280 ELISA Kit, STC1 ELISA Kit, zgc:153307 ELISA Kit, LOC777959 ELISA Kit, stanniocalcin 1 ELISA Kit, stanniocalcin 1 L homeolog ELISA Kit, stanniocalcin 1 S homeolog ELISA Kit, STC1 ELISA Kit, Stc1 ELISA Kit, stc1.L ELISA Kit, stc1.S ELISA Kit, stc1 ELISA Kit, LOC777959 ELISA Kit

Gene ID: 6781

NCBI Accession: NP_003146

UniProt: P52823

Pathways: Hormone Activity