BMP7 ELISA Kit

Catalog No. ABIN6720184

Supplier Product No.: koa0870

Mouse BMP7 ELISA Kit, Colorimetric assay for quantification of Mouse BMP7.

Quick Overview for BMP7 ELISA Kit (ABIN6720184)

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Osseous Tissue, Plasma (EDTA), Plasma (heparin), Serum

Product Details BMP7 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0870

Supplier: Rockland

Purpose: Mouse BMP-7 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: CHO
Immunogen sequence: S292-H430

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: a monoclonal antibody from rat, a biotinylated polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each mouse BMP-7 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.7%, 6.6%, 7.7%
Inter-Assay Precision (CV%): 7.8%, 6.8%, 9.4%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for BMP7

Target: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Alternative Name: BMP-7

Background:

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Gene Full Name: bone morphogenetic protein 7

Gene ID: 12162

NCBI Accession: NP_031583

UniProt: P23359

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance