IL-20 ELISA Kit

Catalog No. ABIN6720199

Supplier Product No.: koa0814

Mouse IL-20 ELISA Kit Colorimetric assay for quantification of Mouse IL-20 in Cell Culture Supernatant, Plasma (EDTA - heparin) and Serum

Quick Overview for IL-20 ELISA Kit (ABIN6720199)

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details IL-20 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0814

Supplier: Rockland

Purpose: Mouse IL-20 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant mouse IL-20. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL-20 standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: E.coli, Immunogen sequence: L25-E176

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for IL-20

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Alternative Name: IL-20

Background:

Synonyms: Cytokine Zcyto10, Four alpha helix cytokine, IL-20, IL10D, IL20, IL20_HUMAN, Interleukin 20, Interleukin-20, MGC96907, UNQ852/PRO1801, ZCYTO10

Background: IL-20, also known as Interleukin 20 or Zcyto10, is a protein that in humans is encoded by the IL20 gene. This gene is mapped to 1q32.1. IL-20 is a protein belonging to the IL-10 family of cytokines. This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Gene ID: 58181

NCBI Accession: NP_001298020

UniProt: Q9JKV9

Pathways: Protein targeting to Nucleus