IL-20 ELISA Kit

Catalog No. ABIN6720229

Product Details IL-20 ELISA Kit

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Cytokine Zcyto10, Four alpha helix cytokine, IL-20, IL10D, IL20, IL20_HUMAN, Interleukin 20, Interleukin-20, MGC96907, UNQ852/PRO1801, ZCYTO10

Background: Interleukin-20(IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Gene Name: IL20

Production: Natural and recombinant human IL-20. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: L25-E176

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IL-20 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-20 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL-20

Target: IL-20 (IL20) (Interleukin 20 (IL20))

Alternative Name: IL-20 (IL20 Products)

Synonyms: IL-20 ELISA Kit, IL10D ELISA Kit, ZCYTO10 ELISA Kit, Zcyto10 ELISA Kit, IL20 ELISA Kit, interleukin 20 ELISA Kit, IL20 ELISA Kit, Il20 ELISA Kit

Gene ID: 50604

NCBI Accession: NP_061194

UniProt: Q9NYY1

Pathways: Protein targeting to Nucleus