S100A9 ELISA Kit

Catalog No. ABIN6720206

Supplier Product No.: koa0662

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse S100A9.

Quick Overview for S100A9 ELISA Kit (ABIN6720206)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Product Details S100A9 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Supplier Product No.: koa0662

Supplier: Rockland

Purpose: Mouse S100A9 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: A2-K113

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse S100A9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse S100A9 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 4.3%, 5.5%, 4.9%
Inter-Assay Precision (CV%): 5.6%, 7%, 5.8%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100A9

Background:

Background: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.

Gene Full Name: S100 calcium binding protein A9 (calgranulin B)

Gene ID: 20202

NCBI Accession: NP_001268781

UniProt: P31725

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins