Azurocidin ELISA Kit

Catalog No. ABIN6720212

Product Details Azurocidin ELISA Kit

Target: Azurocidin (AZU1) (Azurocidin 1 (AZU1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Azu 1, AZU1, Azurocidin, CAP37, CAP7_HUMAN, Cationic antimicrobial protein 37, Cationic antimicrobial protein CAP37, HBP, Heparin binding protein, Heparin-binding protein, HUMAZUR, Neutrophil azurocidin

Background: Azurocidin, also known as cationic antimicrobial protein CAP37 or heparin-binding protein (HBP), is a protein that in humans is encoded by the AZU1 gene. This encoded protein is a member of the serine protease gene family, but it is not a serine proteinase, because the active site serine and histidine residues are replaced. Azurocidin is mapped to 19p13.3. The protein encoded by this gene is an azurophil granule antibiotic protein, with antibacterial activity. It is also an important multifunctional inflammatory mediator. In addition to it, Azurocidin is also a specific chemoattractant for monocytes. It lacks the chemotactic activity for neutrophils and lymphocytes, and this gene is probably responsible for the wave of monocytes that follows the initial wave of PMNs typical of the inflammatory response.

Gene Name: AZU1

Production: Natural and recombinant human Azurocidin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: I27-P248

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Azurocidin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Azurocidin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Azurocidin

Target: Azurocidin (AZU1) (Azurocidin 1 (AZU1))

Alternative Name: Azurocidin (AZU1 Products)

Synonyms: AZAMP ELISA Kit, AZU ELISA Kit, CAP37 ELISA Kit, HBP ELISA Kit, HUMAZUR ELISA Kit, NAZC ELISA Kit, AZU1 ELISA Kit, Azurocidin ELISA Kit, pHBP ELISA Kit, azurocidin 1 ELISA Kit, azurocidin ELISA Kit, AZU1 ELISA Kit, CpipJ_CPIJ007384 ELISA Kit

Gene ID: 566

NCBI Accession: NP_001691

UniProt: P20160