CSF1R ELISA Kit

Catalog No. ABIN6720221

Supplier Product No.: koa0478

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CSF1R.

Quick Overview for CSF1R ELISA Kit (ABIN6720221)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Product Details CSF1R ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Supplier Product No.: koa0478

Supplier: Rockland

Purpose: Human CSF1R - M-CSFR Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human CSF1R. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes:

ELISA_Dilution: 62.5pg/mL-4000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CSF1R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CSF1R standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: I20-E512

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CSF1R/M-CSFR

Background:

Synonyms: C FMS, CD 115, CD115, CD115 antigen, CFMS, Colony stimulating factor 1 receptor, CSF-1 receptor, CSF-1-R, CSF1R_HUMAN, CSFR, FIM2, FMS, FMS proto oncogene, FMS protooncogene, HDLS, M-CSF Receptor, M-CSF-R, Macrophage colony stimulating factor 1 receptor, Macrophage colony-stimulating factor 1 receptor, McDonough feline sarcoma viral (v fms) oncogene homolog, Oncogen FMS, Proto-oncogene c-Fms, V-FMS McDonough feline sarcoma viral oncogen homolog

Background: CSF1R(Colony stimulating factor 1 receptor),also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5(5q32) on the Crick(minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Gene ID: 1436

NCBI Accession: NP_001275634

UniProt: P07333

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization