CXCL11 ELISA Kit

Catalog No. ABIN6720222

Product Details CXCL11 ELISA Kit

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: b R1|Beta R1, C X C motif chemokine 11, C-X-C motif chemokine 11, Chemokine C-X-C motif ligand 11, CXL11_HUMAN, H174, I TAC, Interferon gamma inducible protein 9, Interferon inducible T cell a chemoattractant I TAC, Interferon inducible T cell alpha chemoattractant, IP9, ITAC, SCYB11, SCYB9B, Small inducible cytokine B11, Small inducible cytokine subfamily B member 11, Small inducible cytokine subfamily B member 9B, Small inducible cytokine subfamily B member 11, Small inducible cytokine subfamily B member 9B

Background: Chemokine(C-X-C motif) ligand 11(CXCL11) is a small cytokine belonging to the CXC chemokine family that is also called Interferon-inducible T-cell alpha chemoattractant(I-TAC) and Interferon-gamma-inducible protein 9(IP-9). By PCR analysis of somatic cell hybrids, the CXCL11 gene was mapped to chromosome 4, and using FISH, it was located to 4q21.2. The CXCL11 was strongly induced by IFNG and beta-interferon, but only weakly induced by alpha-interferon. Induction of CXCL11 required expression of STAT1. What's more, it found that monocyte chemotaxis occurs in response to recombinant CXCL11. RT-PCR analysis detected CXCL11 expression in brain tissue from a patient with AIDS dementia and a patient with multiple sclerosis but not in 2 controls.

Gene Name: CXCL11

Production: Natural and recombinant human CXCL11. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: F22-F94

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CXCL11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL11 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CXCL11

Target: CXCL11 (Chemokine (C-X-C Motif) Ligand 11 (CXCL11))

Alternative Name: CXCL11 - I-TAC (CXCL11 Products)

Synonyms: H174 ELISA Kit, I-TAC ELISA Kit, IP-9 ELISA Kit, IP9 ELISA Kit, SCYB11 ELISA Kit, SCYB9B ELISA Kit, b-R1 ELISA Kit, Cxc11 ELISA Kit, I-tac ELISA Kit, Ip9 ELISA Kit, Itac ELISA Kit, Scyb11 ELISA Kit, Scyb9b ELISA Kit, betaR1 ELISA Kit, C-X-C motif chemokine ligand 11 ELISA Kit, chemokine (C-X-C motif) ligand 11 ELISA Kit, CXCL11 ELISA Kit, Cxcl11 ELISA Kit

Gene ID: 6373

NCBI Accession: NP_001289052

UniProt: O14625