IL17D ELISA Kit

Catalog No. ABIN6720252

Product Details IL17D ELISA Kit

Target: IL17D (Interleukin 17D (IL17D))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: IL 17D, IL 22, IL 27, IL-17D, IL-27, IL17D, IL17D_HUMAN, IL27, Interleukin 17D, Interleukin 27, Interleukin-17D, Interleukin-27

Background: Interleukin 17D (IL-17D) is a member of the unique IL-17 family, which share a conserved cysteine motif. It is mapped to 13q12.11. IL-17D regulates cytokine production in endothelial cells and inhibits hemopoiesis in vitro. Purified recombinant IL-17D inhibited myeloid progenitor cell colony formation. IL-17D can enhance endothelial cell production of IL6, IL8, and GMCSF, but not other cytokines. And it is preferentially expressed in skeletal muscle, brain, adipose tissue, heart, lung, and pancreas.

Gene Name: IL17D

Production: Natural and recombinant mouse IL-17D. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A25-R205

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-17D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL-17D standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for IL17D

Target: IL17D (Interleukin 17D (IL17D))

Alternative Name: IL-17D (IL17D Products)

Synonyms: IL-17D ELISA Kit, IL-22 ELISA Kit, IL-27 ELISA Kit, IL27 ELISA Kit, AI462269 ELISA Kit, Il27a ELISA Kit, il17-4 ELISA Kit, zgc:194257 ELISA Kit, zgc:194303 ELISA Kit, IL-27p28 ELISA Kit, Il30 ELISA Kit, p28 ELISA Kit, interleukin 17D ELISA Kit, interleukin 17d ELISA Kit, interleukin 27 ELISA Kit, IL17D ELISA Kit, Il17d ELISA Kit, il17d ELISA Kit, Il27 ELISA Kit

Gene ID: 239114

NCBI Accession: NP_665836

UniProt: Q8K4C4

Pathways: Cellular Response to Molecule of Bacterial Origin