SFTPD ELISA Kit

Catalog No. ABIN6720259

Product Details SFTPD ELISA Kit

Target: SFTPD (Surfactant Protein D (SFTPD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625 pg/mL - 40000 pg/mL

Minimum Detection Limit: 625 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: COLEC 7, COLEC7, Collectin-7, Collectin7, Lung surfactant protein D, PSP D, PSP-D, PSP-D Surfactant protein D, PSPD, Pulmonary surfactant apoprotein, Pulmonary surfactant associated protein D, Pulmonary surfactant associated protein PSP-D, Pulmonary surfactant-associated protein D, SFTP 4, SFTP4, SFTPD, SFTPD_HUMAN, SP D, SP-D, Surfactant associated protein pulmonary 4, Surfactant pulmonary associated protein D

Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

Gene Name: SFTPD

Production: Natural and recombinant human SP-D. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A21-F419

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human SP-D standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 625pg/mL-40,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: SP-D (SFTPD Products)

Synonyms: COLEC7 ELISA Kit, PSP-D ELISA Kit, SFTP4 ELISA Kit, SP-D ELISA Kit, BDE ELISA Kit, BDSD ELISA Kit, HOX4I ELISA Kit, SPD ELISA Kit, AI573415 ELISA Kit, Sftp4 ELISA Kit, surfactant protein D ELISA Kit, homeobox D13 ELISA Kit, surfactant associated protein D ELISA Kit, SFTPD ELISA Kit, HOXD13 ELISA Kit, Sftpd ELISA Kit, SP-D ELISA Kit

Gene ID: 6441

NCBI Accession: NP_003010

UniProt: P35247