REG4 ELISA Kit

Catalog No. ABIN6720301

Supplier Product No.: koa0765

Human REG4 ELISA Kit, Colorimetric assay for quantification of Human REG4.

Quick Overview for REG4 ELISA Kit (ABIN6720301)

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details REG4 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0765

Supplier: Rockland

Purpose: Human REG-4 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: D23-P158

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human REG-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human REG-4 standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 4.6%, 7.5%, 5.4%
Inter-Assay Precision (CV%): 7.1%, 8.2%, 7.1%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for REG4

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Alternative Name: REG-4

Background:

Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.

Gene Full Name: regenerating family member 4

Gene ID: 83998

NCBI Accession: NP_001152824

UniProt: Q9BYZ8