REG4 ELISA Kit

Catalog No. ABIN6720301

Product Details REG4 ELISA Kit

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Gastrointestinal secretory protein, GISP, REG IV, REG like protein, REG-4, REG-like protein, Reg4, REG4_HUMAN, Regenerating gene type IV, Regenerating islet derived family member 4, Regenerating islet derived protein 4 precursor, Regenerating islet-derived protein 4, Regenerating islet-derived protein IV, REGIV, RELP

Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.

Gene Name: REG4

Production: Natural and recombinant human REG-4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: D23-P158

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human REG-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human REG-4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for REG4

Target: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Alternative Name: REG-4 (REG4 Products)

Synonyms: GISP ELISA Kit, REG-IV ELISA Kit, RELP ELISA Kit, 2010002L15Rik ELISA Kit, regenerating family member 4 ELISA Kit, regenerating islet-derived family, member 4 ELISA Kit, Reg4 ELISA Kit, REG4 ELISA Kit

Gene ID: 83998

NCBI Accession: NP_001152824

UniProt: Q9BYZ8