Relaxin 1 ELISA Kit

Catalog No. ABIN6720306

Supplier Product No.: koa0722

The Human Relaxin 1 ELISA Kit (ABIN6720306) is a Colorimetric ELISA Kit designed to quantify Human Relaxin 1.

Quick Overview for Relaxin 1 ELISA Kit (ABIN6720306)

Target: Relaxin 1 (RLN1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Product Details Relaxin 1 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Supplier Product No.: koa0722

Supplier: Rockland

Purpose: Human Relaxin 1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: R162-Cys185 (A) & K26-S53 (B)

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human Relaxin 1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each human Relaxin 1 standard solution and each sample is measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.6%, 4.6%, 4.2%
Inter-Assay Precision (CV%): 7.9%, 5.4%, 4.6%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Relaxin 1

Target: Relaxin 1 (RLN1)

Alternative Name: Relaxin 1

Background:

Background: Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to human chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.

Gene Full Name: relaxin 1

Gene ID: 6013

NCBI Accession: NP_008842

UniProt: P04808