CD93 ELISA Kit

Catalog No. ABIN6720325

Supplier Product No.: koa0792

Human CD93 ELISA Kit, Colorimetric assay for quantification of Human CD93.

Quick Overview for CD93 ELISA Kit (ABIN6720325)

Target: CD93

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine

Product Details CD93 ELISA Kit

Minimum Detection Limit: 78 pg/mL

Supplier Product No.: koa0792

Supplier: Rockland

Purpose: Human CD93 - C1qR Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: NS0
Immunogen sequence: A24-K580

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human CD93 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva or urine to each empty well. We recommend that each human CD93 standard solution and each sample is measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 7.7%, 7.6%, 6.7%
Inter-Assay Precision (CV%): 8.3%, 8.9%, 7.2%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for CD93

Target: CD93

Alternative Name: CD93/C1qR

Background:

Background: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

Gene Full Name: CD93 Molecule

Gene ID: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3