ADIPOQ ELISA Kit

Catalog No. ABIN6720369

Supplier Product No.: koa0797

The Rat ADIPOQ ELISA Kit (ABIN6720369) is a Colorimetric ELISA Kit designed to quantify Rat ADIPOQ.

Quick Overview for ADIPOQ ELISA Kit (ABIN6720369)

Target: ADIPOQ (Adiponectin (ADIPOQ))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine

Product Details ADIPOQ ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0797

Supplier: Rockland

Purpose: Rat Adiponectin Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: I19-N244

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Comment:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. It is recommended that each rat Adiponectin standard solution and each sample be measured in duplicate.

Assay Precision: Intra-Assay Precision (CV%): 5.8%, 7.4%, 4%
Inter-Assay Precision (CV%): 7.4%, 8.2%, 4.1%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: Adiponectin

Background:

Background: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Gene Full Name: adiponectin, C1Q and collagen domain containing

Gene ID: 246253

NCBI Accession: NP_653345

UniProt: Q8K3R4