CD163 ELISA Kit

Catalog No. ABIN6720374

Product Details CD163 ELISA Kit

Target: CD163

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.78 ng/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: CD_antigen=CD163, CD163, Hemoglobin scavenger receptor, M130, MM130, Scavenger receptor cysteine-rich type 1 protein M130

Background: CD163(Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich(SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.

Gene Name: CD163

Production: Natural and recombinant mouse CD163. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V39-T1045

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse CD163 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin) to each empty well. We recommend that each mouse CD163 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for CD163

Target: CD163

Alternative Name: CD163 (CD163 Products)

Synonyms: DMBT1 ELISA Kit, CD163 ELISA Kit, CD163v2 ELISA Kit, CD163v3 ELISA Kit, M130 ELISA Kit, MM130 ELISA Kit, deleted in malignant brain tumors 1 protein-like ELISA Kit, CD163 molecule ELISA Kit, CD163 antigen ELISA Kit, LOC426820 ELISA Kit, CD163 ELISA Kit, Cd163 ELISA Kit

Gene ID: 93671

NCBI Accession: NP_444324

UniProt: Q2VLH6