MST1 ELISA Kit

Catalog No. ABIN6720389

Supplier Product No.: koa0485

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MST1 in Cell Culture Supernatant, Plasma (heparin), Saliva, Serum and Urine.

Quick Overview for MST1 ELISA Kit (ABIN6720389)

Target: MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine

Product Details MST1 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Supplier Product No.: koa0485

Supplier: Rockland

Purpose: Human MSP - MST1 Sandwich ELISA Kit for Quantitative Detection

Brand: AccuSignal™

Analytical Method: Quantitative

Specificity:

Production: Natural and recombinant human MSP. There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Application Details

Application Notes: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MSP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MSP standard solution and each sample be measured in duplicate.

Comment:

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-G711

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for MST1

Target: MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))

Alternative Name: MSP/MST1

Background:

Synonyms: D3F15S2, D3F15S2h, D9H3F15S2, DNF15S2, DNF15S2h, Hepatocyte growth factor like protein, Hepatocyte growth factor like protein homolog, HGFL, Macrophage stimulating 1, Macrophage stimulatory protein, MSP, MST1, NF15S2

Background: Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein(HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3(3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Gene ID: 4485

NCBI Accession: NP_066278

UniProt: P26927