ADA ELISA Kit

Catalog No. ABIN6720392

Product Details ADA ELISA Kit

Target: ADA (Adenosine Deaminase (ADA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Pleural Fluid, Serum, Tissue Homogenate

Analytical Method: Quantitative

Characteristics:

Synonyms: ada, ADA1, ADA 1, Adenosine aminohydrolase, Adenosine deaminase

Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Gene Name: ADA

Production: Natural and recombinant human ADA. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: sf21, Immunogen sequence: A2-L363

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, tissue homogenates or pleural fluid to each empty well. It is recommended that each human ADA standard solution and each sample be measured in duplicate.

ELISA Dilution: 15.6pg/mL-1000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for ADA

Target: ADA (Adenosine Deaminase (ADA))

Alternative Name: ADA (ADA Products)

Synonyms: ADA-like ELISA Kit, xada ELISA Kit, ADA ELISA Kit, CG11994 ELISA Kit, Dmel\\CG11994 ELISA Kit, DrosADA ELISA Kit, dADA ELISA Kit, zgc:92028 ELISA Kit, adenosine deaminase ELISA Kit, adenosine deaminase S homeolog ELISA Kit, Adenosine deaminase ELISA Kit, ADA ELISA Kit, Ada ELISA Kit, ada.S ELISA Kit, ada ELISA Kit

Gene ID: 100

NCBI Accession: NP_000013

UniProt: P00813

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process