HAVCR1 ELISA Kit

Catalog No. ABIN6720396

Product Details HAVCR1 ELISA Kit

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.2 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate, Urine

Analytical Method: Quantitative

Characteristics:

Synonyms: HAVCR, HAVcr-1, Havcr1, HAVR1_HUMAN, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, KIM-1, T cell immunoglobin domain and mucin domain protein 1, T-cell immunoglobulin and mucin domain-containing protein 1, T-cell membrane protein 1, TIM, TIM-1, TIM1, TIMD-1, TIMD1

Background: KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al.(1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al.(2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al.(2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8KDa.

Gene Name: HAVCR1

Production: Natural and recombinant mouse KIM1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Y22-T212

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse KIM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, tissue homogenates, serum, plasma(heparin, EDTA) or urine to each empty well. It is recommended that each mouse KIM1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for HAVCR1

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Alternative Name: KIM1 (HAVCR1 Products)

Synonyms: HAVCR ELISA Kit, HAVCR-1 ELISA Kit, KIM-1 ELISA Kit, KIM1 ELISA Kit, TIM ELISA Kit, TIM-1 ELISA Kit, TIM1 ELISA Kit, TIMD-1 ELISA Kit, TIMD1 ELISA Kit, Kim1 ELISA Kit, HAVCR1 ELISA Kit, LOC100226241 ELISA Kit, AI503787 ELISA Kit, Tim1 ELISA Kit, Timd1 ELISA Kit, hepatitis A virus cellular receptor 1 ELISA Kit, hepatitis A virus cellular receptor 1 homolog ELISA Kit, HAVCR1 ELISA Kit, Havcr1 ELISA Kit, LOC100226241 ELISA Kit

Target Type: Virus

Gene ID: 171283

NCBI Accession: NP_001160103

UniProt: Q3V033