Angiostatin ELISA Kit

Catalog No. ABIN6720398

Product Details Angiostatin ELISA Kit

Target: Angiostatin (ANG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: Ang K1-3

Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED₅₀ =70 nM) relative to kringles 1-4(ED₅₀ = 135 nM).

Gene Name: PLG

Production: Natural and recombinant human Angiostatin K1-3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V98-S355(K1-3)

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiostatin K1-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human serum, plasma or cell culture supernates to each empty well. It is recommended that each human Angiostatin K1-3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Angiostatin

Target: Angiostatin (ANG)

Alternative Name: Angiostatin K1-3 (ANG Products)

Synonyms: ALS9 ELISA Kit, HEL168 ELISA Kit, RNASE4 ELISA Kit, RNASE5 ELISA Kit, LPA ELISA Kit, AI649309 ELISA Kit, Pg ELISA Kit, Ab1-346 ELISA Kit, AI385586 ELISA Kit, Ang1 ELISA Kit, Rnase5 ELISA Kit, Rnase5a ELISA Kit, angiogenin ELISA Kit, plasminogen ELISA Kit, angiogenin, ribonuclease, RNase A family, 5 ELISA Kit, ANG ELISA Kit, PLG ELISA Kit, Plg ELISA Kit, Ang ELISA Kit

Gene ID: 5340

NCBI Accession: NP_000292

UniProt: P00747

Pathways: Lipid Metabolism