APOC1 ELISA Kit

Catalog No. ABIN6730064

This Human APOC1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human APOC1.

Quick Overview for APOC1 ELISA Kit (ABIN6730064)

Target: APOC1 (Apolipoprotein C-I (APOC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details APOC1 ELISA Kit

Purpose: Human ApoC1 ELISA Kit. The SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.

Brand: SpeedELISA

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ApoC1. Other species not determined yet.

Characteristics:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Material not included:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Precision pipettes to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Log-log graph paper or computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Application Details

Assay Time: 3 h

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 µl of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 µl of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment.Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. For prepared reagent storage, see table below.

Expiry Date: 6 months

Target Details for APOC1

Target: APOC1 (Apolipoprotein C-I (APOC1))

Alternative Name: ApoC1

Pathways: Apoptosis