Cathepsin L ELISA Kit

Catalog No. ABIN6730136

Product Details Cathepsin L ELISA Kit

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Cathepsin L ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Cathepsin L.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Cathepsin L (CTSL1 Products)

Synonyms: CATL ELISA Kit, CTSL1 ELISA Kit, MEP ELISA Kit, 1190035F06Rik ELISA Kit, Ctsl1 ELISA Kit, fs ELISA Kit, nkt ELISA Kit, CATHL ELISA Kit, CatL ELISA Kit, Ctsl ELISA Kit, CTSL ELISA Kit, mep ELISA Kit, catl ELISA Kit, ctsl ELISA Kit, cpl-1 ELISA Kit, MGC80629 ELISA Kit, MGC107932 ELISA Kit, LOC663234 ELISA Kit, 26-29-p ELISA Kit, Cp1 ELISA Kit, si:dkey-121a11.6 ELISA Kit, CTSV ELISA Kit, catL ELISA Kit, cb15 ELISA Kit, ctsl1b ELISA Kit, hgg1 ELISA Kit, wu:fb30g09 ELISA Kit, zgc:92089 ELISA Kit, cathepsin L ELISA Kit, cathepsin V ELISA Kit, cathepsin L L homeolog ELISA Kit, cathepsin L1 ELISA Kit, Cathepsin L1 ELISA Kit, Cathepsin L ELISA Kit, testin gene ELISA Kit, cathepsin 12 ELISA Kit, cathepsin Lb ELISA Kit, cathepsin L.1 ELISA Kit, CTSL ELISA Kit, Ctsl ELISA Kit, CTSV ELISA Kit, ctsl.L ELISA Kit, ctsl ELISA Kit, LOC100008830 ELISA Kit, catl1 ELISA Kit, 26-29-p ELISA Kit, catl ELISA Kit, Tsp_11596 ELISA Kit, Testin ELISA Kit, cts12 ELISA Kit, LOC102156614 ELISA Kit, ctslb ELISA Kit, ctsl.1 ELISA Kit

UniProt: Q543M3

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades