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HDAC8 ELISA Kit

HDAC8 Reactivity: Mouse Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6730179
  • Target See all HDAC8 ELISA Kits
    HDAC8 (Histone Deacetylase 8 (HDAC8))
    Reactivity
    • 3
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Mouse Histone Deacetylase 8/HDAC8 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Mouse Histone Deacetylase 8.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all HDAC8 ELISA Kits
    HDAC8 (Histone Deacetylase 8 (HDAC8))
    Alternative Name
    Histone Deacetylase 8 (HDAC8 (HDAC8 Products)
    Synonyms
    HD8 ELISA Kit, CDLS5 ELISA Kit, HDACL1 ELISA Kit, MRXS6 ELISA Kit, RPD3 ELISA Kit, WTS ELISA Kit, 2610007D20Rik ELISA Kit, RGD1562895 ELISA Kit, wu:fd19a02 ELISA Kit, zgc:66196 ELISA Kit, ATHDA8 ELISA Kit, HDA8 ELISA Kit, HISTONE DEACETYLASE 8 ELISA Kit, T27G7.14 ELISA Kit, T27G7_14 ELISA Kit, histone deacetylase 8 ELISA Kit, histone deacetylase 8 ELISA Kit, histone deacetylase 8 L homeolog ELISA Kit, CC1G_05302 ELISA Kit, hdac8 ELISA Kit, HDAC8 ELISA Kit, Hdac8 ELISA Kit, hdac8.L ELISA Kit, HDA08 ELISA Kit
    UniProt
    Q8VH37
    Pathways
    Cellular Glucan Metabolic Process
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