Thimet Oligopeptidase 1 ELISA Kit

Catalog No. ABIN6730298

Product Details Thimet Oligopeptidase 1 ELISA Kit

Target: Thimet Oligopeptidase 1 (THOP1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse THOP1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse THOP1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Thimet Oligopeptidase 1

Target: Thimet Oligopeptidase 1 (THOP1)

Alternative Name: THOP1 (THOP1 Products)

Synonyms: EP24.15 ELISA Kit, MEPD_HUMAN ELISA Kit, MP78 ELISA Kit, TOP ELISA Kit, AI131655 ELISA Kit, AI327041 ELISA Kit, THOP1 ELISA Kit, thop1 ELISA Kit, MGC147297 ELISA Kit, LOC397806 ELISA Kit, mepd ELISA Kit, thimet oligopeptidase 1 ELISA Kit, thimet oligopeptidase 1 L homeolog ELISA Kit, thimet oligopeptidase ELISA Kit, peptidase ELISA Kit, Thimet oligopeptidase ELISA Kit, THOP1 ELISA Kit, Thop1 ELISA Kit, thop1 ELISA Kit, thop1.L ELISA Kit, Tc00.1047053506411.10 ELISA Kit, Tc00.1047053511237.10 ELISA Kit, Noca_4613 ELISA Kit, ANI_1_1400064 ELISA Kit, MBOO_RS10705 ELISA Kit, AOR_1_880174 ELISA Kit, AnaeK_0425 ELISA Kit, MPAL_RS03920 ELISA Kit, Hoch_5061 ELISA Kit, mepd ELISA Kit

UniProt: Q8C1A5