ACLY ELISA Kit

Catalog No. ABIN6730345

Product Details ACLY ELISA Kit

Target: ACLY (ATP Citrate Lyase (ACLY))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ACLY ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ACLY.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ACLY

Target: ACLY (ATP Citrate Lyase (ACLY))

Alternative Name: ACLY (ACLY Products)

Synonyms: ACLY ELISA Kit, DDBDRAFT_0205389 ELISA Kit, DDBDRAFT_0235360 ELISA Kit, DDB_0205389 ELISA Kit, DDB_0235360 ELISA Kit, ACL ELISA Kit, BcDNA:LD21334 ELISA Kit, CG8322 ELISA Kit, DmATPCL ELISA Kit, Dmel\\CG8322 ELISA Kit, anon-WO0140519.179 ELISA Kit, dATPCL ELISA Kit, l(2)01466 ELISA Kit, l(2)k09217 ELISA Kit, n(2)k09217 ELISA Kit, ATPCL ELISA Kit, CLATP ELISA Kit, A730098H14Rik ELISA Kit, AW538652 ELISA Kit, Clatp ELISA Kit, acly ELISA Kit, cb722 ELISA Kit, zgc:92008 ELISA Kit, ATP citrate lyase ELISA Kit, ATP citrate lyase S homeolog ELISA Kit, ATP-citrate synthase ELISA Kit, citrate synthase, mitochondrial ELISA Kit, atp-citrate synthase ELISA Kit, ATP citrate lyase a ELISA Kit, ACLY ELISA Kit, acly.S ELISA Kit, LOC587157 ELISA Kit, acly ELISA Kit, ATPCL ELISA Kit, LOC5564509 ELISA Kit, CpipJ_CPIJ010291 ELISA Kit, Bm1_26245 ELISA Kit, ACL ELISA Kit, Acly ELISA Kit, aclya ELISA Kit

UniProt: P53396

Pathways: Warburg Effect