Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

KLKB1 ELISA Kit

KLKB1 Reactivity: Mouse Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6730355
  • Target See all KLKB1 ELISA Kits
    KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))
    Reactivity
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Mouse KLKB1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Mouse KLKB1.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling KLKB1 ELISA Kit
    Top Product
    Discover our top product KLKB1 ELISA Kit
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all KLKB1 ELISA Kits
    KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))
    Alternative Name
    KLKB1 (KLKB1 Products)
    Synonyms
    KLK3 ELISA Kit, PPK ELISA Kit, APS ELISA Kit, Kal-3 ELISA Kit, Kal3 ELISA Kit, Klk3 ELISA Kit, PSA ELISA Kit, KALP15 ELISA Kit, Kalp1 ELISA Kit, KLKB1 ELISA Kit, DKFZp459J2015 ELISA Kit, klkb1 ELISA Kit, kallikrein B1 ELISA Kit, kallikrein B, plasma 1 ELISA Kit, plasma kallikrein ELISA Kit, Plasma kallikrein ELISA Kit, kallikrein B, plasma (Fletcher factor) 1 ELISA Kit, coagulation factor XI ELISA Kit, kallikrein B1 L homeolog ELISA Kit, KLKB1 ELISA Kit, Klkb1 ELISA Kit, CpipJ_CPIJ004042 ELISA Kit, CpipJ_CPIJ014955 ELISA Kit, CpipJ_CPIJ020108 ELISA Kit, VAA_02266 ELISA Kit, LOC100549617 ELISA Kit, klkb1 ELISA Kit, klkb1.L ELISA Kit
    UniProt
    P26262
    Pathways
    Complement System, Brown Fat Cell Differentiation
You are here:
Support