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PREP ELISA Kit

PREP Reactivity: Mouse Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6730393
  • Target See all PREP ELISA Kits
    PREP (Prolyl Endopeptidase (PREP))
    Reactivity
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Mouse PREP ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Mouse PREP.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all PREP ELISA Kits
    PREP (Prolyl Endopeptidase (PREP))
    Alternative Name
    PREP (PREP Products)
    Synonyms
    PE ELISA Kit, PEP ELISA Kit, AI047692 ELISA Kit, AI450383 ELISA Kit, D10Wsu136e ELISA Kit, Pop ELISA Kit, rPop ELISA Kit, im:7140031 ELISA Kit, zgc:110670 ELISA Kit, prolyl endopeptidase ELISA Kit, Prolyl endopeptidase ELISA Kit, RB12337 ELISA Kit, AM1_4884 ELISA Kit, ppce ELISA Kit, PREP ELISA Kit, Prep ELISA Kit, prep ELISA Kit
    UniProt
    Q9QUR6
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