METAP2 ELISA Kit

Catalog No. ABIN6730395

Product Details METAP2 ELISA Kit

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse METAP2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse METAP2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for METAP2

Target: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Alternative Name: METAP2 (METAP2 Products)

Synonyms: metap2 ELISA Kit, MGC53792 ELISA Kit, METAP2 ELISA Kit, GB13458 ELISA Kit, DDBDRAFT_0190923 ELISA Kit, DDBDRAFT_0304991 ELISA Kit, DDB_0190923 ELISA Kit, DDB_0304991 ELISA Kit, 4930584B20Rik ELISA Kit, A930035J23Rik ELISA Kit, AI047573 ELISA Kit, AL024412 ELISA Kit, AU014659 ELISA Kit, Amp2 ELISA Kit, Mnpep ELISA Kit, p67 ELISA Kit, p67eIF2 ELISA Kit, MAP2 ELISA Kit, MNPEP ELISA Kit, wu:fb98h06 ELISA Kit, zgc:66250 ELISA Kit, methionyl aminopeptidase 2 L homeolog ELISA Kit, methionyl aminopeptidase 2 ELISA Kit, methionine aminopeptidase 2 ELISA Kit, methionine aminopeptidase ELISA Kit, methionyl aminopeptidase 2 S homeolog ELISA Kit, methionyl aminopeptidase 2b ELISA Kit, metap2.L ELISA Kit, METAP2 ELISA Kit, metap2 ELISA Kit, LOC551771 ELISA Kit, CAALFM_C604080WA ELISA Kit, Metap2 ELISA Kit, metap2.S ELISA Kit, metap2b ELISA Kit

UniProt: O08663

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling