Adenylosuccinate Lyase ELISA Kit

Catalog No. ABIN6730398

Product Details Adenylosuccinate Lyase ELISA Kit

Target: Adenylosuccinate Lyase (ADSL)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ADSL ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ADSL.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Adenylosuccinate Lyase

Target: Adenylosuccinate Lyase (ADSL)

Alternative Name: ADSL (ADSL Products)

Synonyms: fi60b04 ELISA Kit, zgc:56061 ELISA Kit, wu:fi60b04 ELISA Kit, MGC53383 ELISA Kit, MGC69239 ELISA Kit, adsL ELISA Kit, F15C21.8 ELISA Kit, F15C21_8 ELISA Kit, AMPS ELISA Kit, ASASE ELISA Kit, ASL ELISA Kit, Adl ELISA Kit, Asl ELISA Kit, purB ELISA Kit, adenylosuccinate lyase ELISA Kit, adenylosuccinate lyase L homeolog ELISA Kit, adenylosuccinase; ASL ELISA Kit, Adenylosuccinate lyase ELISA Kit, L-Aspartase-like family protein ELISA Kit, ADSL ELISA Kit, adsl ELISA Kit, adsl.L ELISA Kit, ASL ELISA Kit, purB ELISA Kit, purB,adsL ELISA Kit, Mrub_2293 ELISA Kit, MMAH_RS02975 ELISA Kit, Arnit_0089 ELISA Kit, Mesil_1058 ELISA Kit, Slip_0597 ELISA Kit, Trad_2452 ELISA Kit, Deba_0652 ELISA Kit, Toce_1174 ELISA Kit, Acear_2324 ELISA Kit, Igag_0272 ELISA Kit, Saut_2156 ELISA Kit, Fbal_1996 ELISA Kit, Ndas_2434 ELISA Kit, Ilyop_0196 ELISA Kit, Lbys_2961 ELISA Kit, MFER_RS02920 ELISA Kit, Palpr_2192 ELISA Kit, Calni_0061 ELISA Kit, Ftrac_3746 ELISA Kit, AT1G36280 ELISA Kit, Olsu_1764 ELISA Kit, Adsl ELISA Kit

UniProt: P30566

Pathways: Ribonucleoside Biosynthetic Process