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DDC ELISA Kit
DDC
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details DDC ELISA Kit
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Target
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DDC
(Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))
Reactivity
All reactivities for DDC elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse DDC ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse DDC.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for DDC
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Target
See all DDC ELISA Kits
DDC
(Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))
Alternative Name
DDC (DDC Products )
Synonyms
AADC ELISA Kit, wu:fa56d05 ELISA Kit, wu:fd59h03 ELISA Kit, wu:fk20h01 ELISA Kit, zgc:65801 ELISA Kit, zgc:76929 ELISA Kit, Ddc ELISA Kit, DdcDv ELISA Kit, Dvir\\GJ17995 ELISA Kit, GJ17995 ELISA Kit, dvir_GLEANR_2561 ELISA Kit, Aadc ELISA Kit, 5-HT ELISA Kit, CG10697 ELISA Kit, DDC ELISA Kit, DdcDm ELISA Kit, Dmel\\CG10697 ELISA Kit, ddc ELISA Kit, fDDC ELISA Kit, l(2)37Bl ELISA Kit, l(2)37Ch ELISA Kit, l(2)k02104 ELISA Kit, dopa decarboxylase ELISA Kit, Dopa-decarboxylase ELISA Kit, dopa decarboxylase (aromatic L-amino acid decarboxylase) ELISA Kit, Dopa decarboxylase ELISA Kit, dopa decarboxylase L homeolog ELISA Kit, DDC ELISA Kit, ddc ELISA Kit, Ddc ELISA Kit, Dvir\Ddc ELISA Kit, ddc.L ELISA Kit
UniProt
O88533
Pathways
Dopaminergic Neurogenesis
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