Acetylcholinesterase ELISA Kit

Catalog No. ABIN6730408

Product Details Acetylcholinesterase ELISA Kit

Target: Acetylcholinesterase (AChE)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human Acetylcholinesterase/ACHE ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Acetylcholinesterase.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase (ACHE (AChE Products)

Synonyms: ACEE ELISA Kit, ARACHE ELISA Kit, N-ACHE ELISA Kit, YT ELISA Kit, Ache ELISA Kit, GB14873 ELISA Kit, zgc:92550 ELISA Kit, ACE ELISA Kit, Dsim\\GD20515 ELISA Kit, GD20515 ELISA Kit, dsim_GLEANR_4292 ELISA Kit, ache ELISA Kit, mE1a ELISA Kit, mE1b ELISA Kit, mE1c ELISA Kit, mE1c-long ELISA Kit, mE1d ELISA Kit, mE1d' ELISA Kit, mE1e ELISA Kit, arache ELISA Kit, n-ache ELISA Kit, acetylcholinesterase (Cartwright blood group) ELISA Kit, acetylcholinesterase 2 ELISA Kit, acetylcholinesterase ELISA Kit, Acetylcholine esterase ELISA Kit, acetylcholinesterase (Cartwright blood group) L homeolog ELISA Kit, collagen type I alpha 2 chain ELISA Kit, ACHE ELISA Kit, AChE-2 ELISA Kit, Ache ELISA Kit, ache ELISA Kit, Dsim\Ace ELISA Kit, ache.L ELISA Kit, COL1A2 ELISA Kit, ACE-1 ELISA Kit

UniProt: P22303

Pathways: Skeletal Muscle Fiber Development