Cofilin ELISA Kit

Catalog No. ABIN6730455

Product Details Cofilin ELISA Kit

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CFL1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human CFL1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: CFL1 (CFL1 Products)

Synonyms: cfl1 ELISA Kit, xac1 ELISA Kit, xac2 ELISA Kit, cfl ELISA Kit, cb86 ELISA Kit, sb:cb86 ELISA Kit, zgc:56501 ELISA Kit, wu:fb18a04 ELISA Kit, wu:fk77b03 ELISA Kit, 12/8 ELISA Kit, ADF ELISA Kit, ADF/cofilin ELISA Kit, Adf ELISA Kit, CG4254 ELISA Kit, Cadf ELISA Kit, D-cof ELISA Kit, D61 ELISA Kit, Dmel\\CG4254 ELISA Kit, T8 ELISA Kit, Tsr ELISA Kit, cofilin ELISA Kit, cofilin/ADF ELISA Kit, cofilin/actin depolymerizing factor homolog ELISA Kit, l(2)03101 ELISA Kit, l(2)k03101 ELISA Kit, l(2)k05633 ELISA Kit, l(2)k13409 ELISA Kit, ntf ELISA Kit, CFL ELISA Kit, AA959946 ELISA Kit, Cof ELISA Kit, CFL1 ELISA Kit, cofilin 1 (non-muscle) L homeolog ELISA Kit, cofilin 1 (non-muscle) ELISA Kit, cofilin 1 (non-muscle), like ELISA Kit, cofilin 1 ELISA Kit, twinstar ELISA Kit, cofilin ELISA Kit, cofilin 1, non-muscle ELISA Kit, Contrafreeloading ELISA Kit, cfl1.L ELISA Kit, cfl1 ELISA Kit, cfl1l ELISA Kit, CFL1 ELISA Kit, tsr ELISA Kit, COF1 ELISA Kit, TSTA_042790 ELISA Kit, Cfl1 ELISA Kit, CFL ELISA Kit

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events