TRH ELISA Kit

Catalog No. ABIN6730541

This Rat, Human and Mouse TRH ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat, Human and Mouse TRH.

Quick Overview for TRH ELISA Kit (ABIN6730541)

Target: TRH (Thyrotropin-Releasing Hormone (TRH))

Reactivity: Rat, Human, Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum

Product Details TRH ELISA Kit

Minimum Detection Limit: 0.1 ng/mL

Purpose: Human/Mouse/Rat Thyrotropin-releasing hormone EIA Kit optimized for serum and cell culture media. Competition-based ELISA on a 96-well strip plate.

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat Thyrotropin-releasing hormone

Sensitivity: 0.6 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samplesHuman: 8x / Mouse: 8x / Rat: 8x

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for TRH

Target: TRH (Thyrotropin-Releasing Hormone (TRH))

Alternative Name: Thyrotropin-releasing hormone

Gene ID: 7200

UniProt: P20396

Pathways: Positive Regulation of Peptide Hormone Secretion, Feeding Behaviour