FZD2 ELISA Kit

Catalog No. ABIN6730675

Product Details FZD2 ELISA Kit

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse Frizzled-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse Frizzled-2.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: Frizzled-2 (FZD2 Products)

Synonyms: BG01711 ELISA Kit, CG9739 ELISA Kit, D-Fz2 ELISA Kit, D-fz2 ELISA Kit, DFZ2 ELISA Kit, DFz-2 ELISA Kit, DFz2 ELISA Kit, Dfrz2 ELISA Kit, Dfz2 ELISA Kit, Dfz[[2]] ELISA Kit, Dm Fz2 ELISA Kit, Dmel\\CG9739 ELISA Kit, FZ2 ELISA Kit, Fz2 ELISA Kit, dFz2 ELISA Kit, dfz(2) ELISA Kit, dfz2 ELISA Kit, dfz2r ELISA Kit, frz2 ELISA Kit, fz-2 ELISA Kit, fz8 ELISA Kit, zfz2 ELISA Kit, zg08 ELISA Kit, cb383 ELISA Kit, wu:fb70g09 ELISA Kit, FZD2 ELISA Kit, fzE2 ELISA Kit, hFz2 ELISA Kit, Fz-10 ELISA Kit, Fzd10 ELISA Kit, cFz-2 ELISA Kit, frizzled2 ELISA Kit, fz2 ELISA Kit, xfz2 ELISA Kit, AL033370 ELISA Kit, AW456835 ELISA Kit, Fz10 ELISA Kit, Mfz10 ELISA Kit, Mfz10a ELISA Kit, frizzled 2 ELISA Kit, frizzled class receptor 2 ELISA Kit, frizzled class receptor 2 S homeolog ELISA Kit, fz2 ELISA Kit, fzd2 ELISA Kit, FZD2 ELISA Kit, Fzd2 ELISA Kit, fzd2.S ELISA Kit

UniProt: Q9JIP6

Pathways: WNT Signaling