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Galectin 2 ELISA Kit
LGALS2
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details Galectin 2 ELISA Kit
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Target
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Galectin 2 (LGALS2)
(Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))
Reactivity
All reactivities for Galectin 2 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse Galectin-2 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse Galectin-2.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for Galectin 2
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Target
See all Galectin 2 (LGALS2) ELISA Kits
Galectin 2 (LGALS2)
(Lectin, Galactoside-Binding, Soluble, 2 (LGALS2))
Alternative Name
Galectin-2 (LGALS2 Products )
Synonyms
LGALS3 ELISA Kit, galectin-2 ELISA Kit, HL14 ELISA Kit, 2200008F12Rik ELISA Kit, AI324147 ELISA Kit, LGALS2 ELISA Kit, hl14 ELISA Kit, Drgal1-L3 ELISA Kit, lgals1l3 ELISA Kit, zgc:66283 ELISA Kit, Drgal1-L2 ELISA Kit, lgals1l2 ELISA Kit, zgc:86752 ELISA Kit, galectin 2 ELISA Kit, lectin, galactose-binding, soluble 2 ELISA Kit, lectin, galactoside binding soluble 2 ELISA Kit, lectin, galactoside-binding, soluble, 2b ELISA Kit, lectin, galactoside-binding, soluble, 2a ELISA Kit, LGALS2 ELISA Kit, Lgals2 ELISA Kit, lgals2 ELISA Kit, lgals2b ELISA Kit, lgals2a ELISA Kit
UniProt
Q9CQW5
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