P4HB ELISA Kit

Catalog No. ABIN6730763

Product Details P4HB ELISA Kit

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Mouse P4HB ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse P4HB.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: P4HB (P4HB Products)

Synonyms: DSI ELISA Kit, ERBA2L ELISA Kit, GIT ELISA Kit, P4Hbeta ELISA Kit, PDI ELISA Kit, PDIA1 ELISA Kit, PHDB ELISA Kit, PO4DB ELISA Kit, PO4HB ELISA Kit, PROHB ELISA Kit, PDA2 ELISA Kit, PDIP ELISA Kit, PDIR ELISA Kit, ERp59 ELISA Kit, Pdia1 ELISA Kit, Thbp ELISA Kit, p55 ELISA Kit, XPDIp ELISA Kit, pdi ELISA Kit, 1810041F13Rik ELISA Kit, AI661267 ELISA Kit, Pdip ELISA Kit, Pdipl ELISA Kit, p58 ELISA Kit, prolyl 4-hydroxylase subunit beta ELISA Kit, protein disulfide isomerase family A member 2 ELISA Kit, prolyl 4-hydroxylase, beta polypeptide ELISA Kit, protein disulfide isomerase family A member 2 L homeolog ELISA Kit, protein disulfide isomerase associated 2 ELISA Kit, prolyl 4-hydroxylase subunit beta L homeolog ELISA Kit, protein disulfide-isomerase ELISA Kit, P4HB ELISA Kit, PDIA2 ELISA Kit, P4hb ELISA Kit, pdia2.L ELISA Kit, Pdia2 ELISA Kit, p4hb.L ELISA Kit, LOC8281065 ELISA Kit

UniProt: P09103

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism