DKK2 ELISA Kit

Catalog No. ABIN6730808

The Mouse DKK2 ELISA Kit (ABIN6730808) is a Colorimetric ELISA Kit designed to quantify Mouse DKK2.

Quick Overview for DKK2 ELISA Kit (ABIN6730808)

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.16 ng/mL - 60 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details DKK2 ELISA Kit

Minimum Detection Limit: 0.16 ng/mL

Purpose: Mouse Dkk-2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BTLA, BTN1A1, C1ra, Cathepsin L, CD200 R1, CD2F-10, CD38, Cerberus 1, CRISP-4, CXCL17, DCC, DcTRAIL R2, EG-VEGF, EPCR, EphA3, EphA4, Ephrin-B1, EphrinB2, Frizzled-2, Galectin-4, Galectin-9, GDF-5, GDF-9, GFR alpha -2, GHR, HIN-1, ICAM-5, IGFBP-L1, IL-10 R alpha, IL17 RD, IL-22 R alpha 1, IL-3 R alpha, JAM-B, Latexin, LILRC1, Matrilin-4, Netrin-1, Netrin-4, Noggin.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 2 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for DKK2

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Alternative Name: Dkk-2

Background:

Gene Symbols: DKK2

Protein Name (Synonyms): Dickkopf-related protein 2 (Dickkopf-2) (Dkk-2) (mDkk-2)

UniProt: Q9QYZ8

Pathways: WNT Signaling