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MST1 ELISA Kit
MST1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Product Details MST1 ELISA Kit
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Target
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MST1
(Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))
Reactivity
All reactivities for MST1 elisa kits
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Mouse MSP ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Mouse MSP.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for MST1
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Target
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MST1
(Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))
Alternative Name
MSP (MST1 Products )
Synonyms
D3F15S2 ELISA Kit, DNF15S2 ELISA Kit, HGFL ELISA Kit, MSP ELISA Kit, NF15S2 ELISA Kit, E2F2 ELISA Kit, D3F15S2h ELISA Kit, D9H3F15S2 ELISA Kit, DNF15S2h ELISA Kit, Hgfl ELISA Kit, HGF1/MSP ELISA Kit, E1A ELISA Kit, XHL ELISA Kit, d3f15s2 ELISA Kit, dnf15s2 ELISA Kit, hgfl ELISA Kit, msp ELISA Kit, mst1 ELISA Kit, nf15s2 ELISA Kit, macrophage stimulating 1 ELISA Kit, macrophage stimulating 1 (hepatocyte growth factor-like) ELISA Kit, serine/threonine kinase 4 ELISA Kit, macrophage stimulating 1 L homeolog ELISA Kit, MST1 ELISA Kit, Mst1 ELISA Kit, STK4 ELISA Kit, mst1.L ELISA Kit
UniProt
P26928
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