IGFBPL1 ELISA Kit

Catalog No. ABIN6810492

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse IGFBPL1 in Cell Culture Supernatant, Cell Samples, Plasma, Serum and Tissue Lysate.

Quick Overview for IGFBPL1 ELISA Kit (ABIN6810492)

Target: IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Product Details IGFBPL1 ELISA Kit

Purpose: Mouse IGFBP-L1 ELISA Kit.

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Mouse IGFBP-L1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for IGFBPL1

Target: IGFBPL1 (Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBPL1))

Alternative Name: IGFBP-L1

UniProt: Q80W15