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IL1RL2 ELISA Kit

IL1RL2 Reactivity: Mouse Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Catalog No. ABIN6810494
  • Target See all IL1RL2 ELISA Kits
    IL1RL2 (Interleukin 1 Receptor-Like 2 (IL1RL2))
    Reactivity
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Mouse IL-1 Rrp2/IL-1 R6 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Mouse IL-1 Rrp2.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Expiry Date
    6 months
  • Target See all IL1RL2 ELISA Kits
    IL1RL2 (Interleukin 1 Receptor-Like 2 (IL1RL2))
    Alternative Name
    IL-1 Rrp2 (IL1RL2 Products)
    Synonyms
    IL-1Rrp2 ELISA Kit, IL-36R ELISA Kit, IL1R-rp2 ELISA Kit, IL1RRP2 ELISA Kit, AI481289 ELISA Kit, AW551444 ELISA Kit, interleukin 1 receptor like 2 ELISA Kit, interleukin 1 receptor-like 2 ELISA Kit, IL1RL2 ELISA Kit, Il1rl2 ELISA Kit
    UniProt
    Q9ERS7
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